粪便DNA小量提取试剂盒

特点：

• 节约时间：60分钟内。无需酚/氯仿抽提。

• 高纯度：样品中无腐殖酸，多糖，苯酚，酶抑制剂。

• 样品大小：50-200mg的新鲜或者冷冻的粪便样品。

• 类型：吸附柱

说明：

• 样品： 50-200mg的新鲜或者冷冻的粪便样品。

• 结合力：30ug的基因组DNA

• 操作时间：60min。

Description 

The FavorPrep Stool DNA Isolation Kit is designed for isolation of high quality total DNA from 50~200mg of fresh or frozen stool sample. The inhibitors of the downstream application such as polysaccharides, humic acid, phenolic compounds will be removed by utilizing the DNA binding column and the sequent buffer system in this kit. The entire procedure is not required the phenol-chloroform extraction and can be finished within 60 min. The purified DNA is ready for PCR and other downstream applications.

Features
•Time Saving: Rapid isolation of ready-to-use DNA within 60 minutes without phenol/chloroform extraction. 
•High purity: Eliminate humic acid, polysaccharides, phenol compounds, and enzyme inhibitor from stool sample. 
•Sample Size: 50~200 mg of fresh or frozen stool sample. 
•Format: Spin Column

Applications
PCR, Real-Time PCR 
Restriction digestion
Hybridization
Disease research

Procedure 
In this simple and rapid process, the stool sample are homogenized and lysed by the buffer containing glass beads, Proteinase K and detergents. Provided special buffer will remove debris, proteins, and polysaccharides by precipitation and other contaminants are washed away by alcohol containing wash buffer. Finally, the purified DNA is eluted by low-salt elution buffer orwater.

Storage Conditions

Stable for 2 year at room temperature 15 -25°C . The Proteinase K Powder store at -20 ℃ .