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血液/培养的细胞总RNA小量提取试剂盒
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血液/培养的细胞总RNA小量提取试剂盒

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商品描述

Description 
The Blood/Cultured Cell Total RNA Purification Mini Kit is designed for purification of total RNA from cultured cells and fresh whole blood using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 300μl of blood within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction.



Features
•  High Purity: OD260/280: 1.9-2.1 
•  High Speed: Rapid speed for the purification high quality RNA from samples ranging in size from 200 μl of blood or from 106 of cells in about 30 minutes. 
•  SafeUse: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.
  Scalable protocol: Solution based procedure can be scaled to suit the application.

Time Required
About 30-60 minutes.

Applications 
•  Nothern blotting hybridizations
•  Primer extension
•  RT-PCR 
•  RNase protection assays
•  Differential display
•  As starting material for purification of mRNA for cDNA synthesis

Sample Source
Whole Blood/Buffy Coat; Cultured Animal Cells, Gram –ve/+ve Bacteria

Sample Size 
Up to 1x 107 animal cultured cells, 1x109 bacterial cells, or 300μl blood.

Yield of Purification

Sample
Size
RNA Yield
Bacterial Cells
1x109
30~50
Cultured Cells
1x106
5~20
Blood
200μl
4~12

Procedure

 

The method uses detergents and a chaotropic salt to lysis cell and inactivate RNase, then RNA in chaotropic salt is bonded to the glass fiber matrix of column. After washing off the contaminants, the purified RNA is eluted by RNase-free water. The entire procedure can be completed about 20~30 minutes.

Storage Conditions
FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit can be stored at room temperature (15-25 ℃ ). Stable for 1 year at room temperature at 15-25℃.

Ordering Information
Cat. No. Product Name Size Kit Contents Store at Protocol

FABRK 001

 

FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit

50 preps.

RL Buffer 
FARB Buffer 
Wash Buffer 1 
Wash Buffer 2 (conc.) 
RNase-Free Water 
FARB Columns 
2ml collection tubes 
Filter Column 

Store at room temperature at 15-25 ℃ for 1 year.

FABRK 001-1

100 preps

FABRK 001-2

300 preps

FABRK 003

 

FavorPrep™ Blood/Cultured Cell Total RNA Maxi Kit

10 preps.

RL Buffer 
FARB Buffer 
Wash Buffer 1 
Wash Buffer 2 (conc.) 
RNase-Free Water 
FARB Columns 
Collection tubes 
Filter Column

Store at room temperature at 15-25 ℃ for 1 year.

FABRK 003-1

24 preps

 

血液总
RNA小量提取试剂盒(血液/培养的细胞)


 

特点:

l  高纯度:OD 260/280:1.9-2.1

l  安全使用:该试剂盒使用离心柱去掉细胞中的蛋白和核酶,无需酚/氯仿抽提。

l  节约时间:30-60分钟(取决于样品的类型)。

 

说明:

l  样品:0.3ml的新鲜全血

                 1X107动物培养细胞

                 1X108细菌培养细胞

                 5 X107酵母

l  结合力:100ugRNA

l  预期产量:血液样品 4-12ug  培养的细胞 10-65ug

l  操作时间:30-60min