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血液基因组DNA提取试剂盒
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血液基因组DNA提取试剂盒

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商品描述

Genomic DNA Extraction Blood DNA Mini / Maxi Kit


Description 
The FavorPrep™ Blood Genomic DNA Extraction Kit is designed for rapid extraction of pure genomic DNA from whole blood, serum, buffy coat, body fluids, lymphocytes and cultured cell. As a column-type tube is utilized in the purification process in three simple steps of binding, washing and then elution for the safe and convenient extraction of high-purity genomic DNA.



Features
•  Extraction of genomic DNA from whole blood, plasma, serum, buffy coat, body fluids, 
lymphocytes and cultured cells.
•  Purified DNA is ready for downstream application such as PCR, Southern blotting.
•  Centrifugation-based method.
•  Efficiently remove cellular debair and inhibitors
•  No phenol/chloroform extraction and ethanol precipitation required.


Applications
PCR
Southern Blotting
Medicolegal Analysis


Sampling
Up to 200 μl of whole blood (with anti-coagulant), plasma, serum, buffy coat, or body fluids; up to 5x105lymphocytes or cultured cells in 200μl PBS.


Yield
About 5μg of total DNA from 200μl of human whole blood; up to 50μg of total DNA, depends on the sample types and the number of cells in the sample.


Yield of Purification

Sample

Amounts

Yield

Whole Blood

200 μl

3~6μg

Whole Blood

1 ml

10~40μg

Buffy Coat

200 μl

30~60μg

Cultured Cells

5*106

15 ~30μg

Bacterial Cells

2*109

100~300μg

The yield of genomic DNA is variable depending on the sample types.


Handling Time
About 60 minutes depending upon the sample types.

 


Storage Conditions
Stable for 2 year at room temperature. The Proteinase K Powder store at -20℃.

Ordering Information

Cat. No.

Product Name

Size

Kit Components

Store at

Protocol

FABGK 001

 

FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit

 

50 preps.

Proteinase K Powder 
FATL Buffer
FABG Buffer
W1 Buffer
Wash Buffer (conc.) 
Elution Buffer 
FABG Mini Columns 
2ml Collection tubes
1.5ml Elution tubes

Store at room temperature for 2 year. (Except Proteinase K Powder, store at -2 0℃.)


FABGK 001-1

100 preps.

FABGK 100

 

FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit

 

100 preps.

RBC Lysis Buffer 
FATL Buffer
FABG Buffer
W1 Buffer
Wash Buffer (conc.) 
Elution Buffer 
FABG Mini Columns 
2ml Collection tubes

Store at room temperature for 2 year. (Except Proteinase K Powder, store at -2 0℃.)


FABGK 300

300 preps.

 

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