Genomic DNA Extraction Blood DNA Mini / Maxi Kit
Description
The FavorPrep™ Blood Genomic DNA Extraction Kit is designed for rapid extraction of pure genomic DNA from whole blood, serum, buffy coat, body fluids, lymphocytes and cultured cell. As a column-type tube is utilized in the purification process in three simple steps of binding, washing and then elution for the safe and convenient extraction of high-purity genomic DNA.
Features
• Extraction of genomic DNA from whole blood, plasma, serum, buffy coat, body fluids,
lymphocytes and cultured cells.
• Purified DNA is ready for downstream application such as PCR, Southern blotting.
• Centrifugation-based method.
• Efficiently remove cellular debair and inhibitors
• No phenol/chloroform extraction and ethanol precipitation required.
Applications
PCR
Southern Blotting
Medicolegal Analysis
Sampling
Up to 200 μl of whole blood (with anti-coagulant), plasma, serum, buffy coat, or body fluids; up to 5x105lymphocytes or cultured cells in 200μl PBS.
Yield
About 5μg of total DNA from 200μl of human whole blood; up to 50μg of total DNA, depends on the sample types and the number of cells in the sample.
Yield of Purification | ||
Sample | Amounts | Yield |
Whole Blood | 200 μl | 3~6μg |
Whole Blood | 1 ml | 10~40μg |
Buffy Coat | 200 μl | 30~60μg |
Cultured Cells | 5*106 | 15 ~30μg |
Bacterial Cells | 2*109 | 100~300μg |
The yield of genomic DNA is variable depending on the sample types. |
Handling Time
About 60 minutes depending upon the sample types.
Storage Conditions
Stable for 2 year at room temperature. The Proteinase K Powder store at -20℃.
Ordering Information | |||||
Cat. No. | Product Name | Size | Kit Components | Store at | Protocol |
FABGK 001 |
FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit
| 50 preps. |
Proteinase K Powder | Store at room temperature for 2 year. (Except Proteinase K Powder, store at -2 0℃.) | |
FABGK 001-1 | 100 preps. | ||||
FABGK 100 |
FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit
| 100 preps. |
RBC Lysis Buffer | Store at room temperature for 2 year. (Except Proteinase K Powder, store at -2 0℃.) | |
FABGK 300 | 300 preps. |
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